The Andersson lab aims to understand how specialized organs systems develop the correct cellular identity and architecture. As developmental biologists, we are interested in why and how genetic mutations lead to specific defects in embryogenesis. We develop new methods to analyze organ architecture, or new technologies to manipulate embryogenesis in mice, with emphasis on the 3Rs. We are focused on the Notch-related disorder Alagille syndrome, a congenital disorder affecting development of several organ systems including the liver and vasculature. In addition to the technologies we are developing, we use mouse models, 3D cell culture, CRISPR cell lines, single cell omics, and patient samples to address fundamental questions with relevance for human health.
Selected Recent Publications (full list)
Andersson Lab May 2022 (Photo by Linda Lindell). First row (sitting, left to right): Bettina Semsch, Elisabeth Verboven, Sandra De Haan, Noemi Van Hul, Katrin Mangold (alumnus, defended PhD Student). Second Row (standing): David Kosek, Afshan Iqbal, Lenka Belicova, Paulina Zydowicz-Machtel, Jia Sun, Emma R Andersson, Lukas Poppe, Jingyan He, Agustin Corbat
Congratulations David Kosek on the publication of your first first-author paper in Nucleic Acids Research! Understanding the rules of miRNA-mRNA target engagement and functional outcomes are essential for refinement of miRNAs or siRNAs as therapeutic tools. David, a PhD student in the Andersson lab who is part of a tight KAW-funded collaboration with the Petzold lab, has studied the impact of miR binding capacities on target downregulation. David uncovered a function for 3′ pairing by the miRNA in rendering mRNA target sites less sensitive to seed-site occlusion in the native mRNA structure. For more, check out the publication online here!
Congratulations to our PhD student Afshan Iqbal, who was awarded the prize for Best Basic Science Poster at the EASL Congress!
Our lab submitted 4 abstracts to the upcoming EASL Congress, three of which were ranked in the Top 10% and competed for Best Poster Prizes (the fourth abstract was an update of an abstract that was previously top-ranked at an EASL congress). Afshan Iqbal, Dr. Elisabeth Verboven, Linde Sevenants & Dr. Noemi Van Hul (co-presenting a poster) and Jingyan He presented our latest insights into bile duct de novo growth in a model of Alagille syndrome using hilar or peripheral biliary organoids, developmental hepatic innervation processes, spatial transcriptomics of the atypical Alagille syndrome fibrotic response, and a new technique to manipulate hepatic stellate cells in utero.
Banijamali et al published in RNA! In this first collaborative publication with the Petzold lab, we report the further development of selective 2′-hydroxyl acylation analyzed by primer extension (SHAPE) to resolve how microRNA-34a (miR-34a) binds its mRNA target, the silent information regulator 1 (mSIRT1), both with and without the Argonaute protein. This is the first paper from our collaboration that was seeded in 2016, in which we ultimately aim to resolve miR:mRNA selectivity principles in vivo, using our lab’s technique NEPTUNE. More papers in the pipeline, coming soon!
Hankeova et al published in EMBO Molecular Medicine and featured on the cover! Simona Hankeova (former PhD student in the lab, now post doc at Genentech), addressed why patients with Alagille syndrome experience spontaneous bleeds, using our mouse model, patient retinographs, and a systematic literature review. With thanks to Noemi Van Hul and Eli Verboven who championed the revision process and ensured that the paper was finalised. Fantastic collaboration with clinicians, scientists worldwide, and over many years. Thank you to the patients for participating in the research process. And thank you to the constructive reviewers through the Review Commons process, an excellent system!
The Sweden Liver Week – Highlight of Swedish Hepatology!
Emma gave the State of the Art Lecture on June 15th.
The Sweden Liver Week is ongoing and three labmembers were selected to give talks: congratulations to Jingyan He, Paulina Zydowicz-Machtel and Lukas Poppe! And a massive thank you to the co-organizers, which include our very own Senior Scientist Noemi Van Hul (abstract selection process was independent of Noemi 😉 )
Congratulations to Jingyan He, whose presentation was selected as Best Talk on Day 1!
And Congratulations to Lukas Poppe, Master’s student in our lab supervised by Noemi Van Hul, whose presentation was selected as Best Talk on Day 2!
Congratulations to Dr Katrin Mangold who successfully defended her PhD!
Dr Mangold is the second PhD graduate from our lab. Her work focused on developing NEPTUNE to target nervous system with high efficiency, and develop it for neural and ectoderm-derived tissues.
Our review on “Modern therapeutic approaches to liver-related disorders“, led by Pavel Strnad (University Hospital RWTH Aachen, Germany) together with Antoine Gardin, Katharina Remih and Emmanuel Gonzales, is out in Journal of Hepatology! We discuss challenges and opportunities in siRNA, modified RNA, gene engineering etc to treat liver disease and diseases stemming from liver dysfunction.
We welcome our new postdoc Agustin Corbat, who performed his PhD in the Quantum Electronics Lab under the direction of Dr. Hernan Grecco at the Department of Physics of the Exact and Natural Sciences Faculty of the University of Buenos Aires, Argentina.
Lenka Belicova (left) and Elisabeth Verboven (right) both awarded Marie Curie fellowships in the EU Horizon MSCA Postdoctoral Fellowships 2021 call! Lenka will use barcode lineage tracing to map liver cell plasticity (Mangold et al, Cell Reports Methods 2021, Kameneva, Artemov et al Nature Genetics 2021) and Eli will investigate the role of the nervous system in liver health and disease. Thank you to the reviewers for seeing the potential in these projects!
Editorial by ER Andersson out now in Hepatology. This editorial discusses exciting new work from Duc Dong’s lab, in the context of previous findings from Stacey Huppert and Holger Willenbring’s labs.
In order to disseminate neural plate targeting with in utero nano-injection (NEPTUNE) (Mangold et al Cell Reports Methods 2021), we are happy to present the full protocol in the Journal of Visualized Experiments (JoVE): Mangold et al, Jove 2022
Also, if you are interested in NEPTUNE or in utero genetic manipulation for your experiments, we set up a core facility to provide NEPTUNE as a service to researchers; INFINiGENE (INFrastructure for in utero GENEtic manipulation).
Our Special Issue of the European Journal of Medical Genetics “Advances in genetic, epigenetic and environmental aspects of rare liver diseases” is out. ER Andersson co-guest-edited this Special Collection together with Prof Dr Ansgar Lohse, on behalf of the European Reference Network for Rare Liver Disease (for which ER Andersson is the External Scientific Advisor). The collection highlights progress in rare diseases such as PSC, PFIC, Wilsons disease and more, and includes an editorial introduction that you can read here.
Collaborative paper led by Franziska Hildebrandt in Johan Ankarklev’s lab at Stockholm University published in Nature Communications, describing the application of spatial transcriptomics to large liver tissue slices. We confirm liver zonation principles and demonstrate how this will be useful to further studies of liver architecture.
Congratulations to Elisabeth Verboven for being awarded a David and Astrid Hagelen Foundation Fellowship for her postdoctoral project! This fellowship will support Dr Verboven for two years, and we are grateful to the foundation and reviewers for finding the project and Dr Verboven meritorious!
Congratulations to Sandra De Haan for being awarded two travel grants to present her work at the ARO 2022 MidWinter Meeting in California! Sandra will present her work on the inner ear development and homeostasis in a mouse model for Alagille syndrome.
Emma R Andersson is awarded a Karolinska Institutet Consolidator Grant! We thank both the internal and external reviewers!
Andersson lab is awarded a 3R Grant from the Swedish Research Council to continue their work developing in utero nano-injection as an alternative method to traditional mouse genetics – aiming to reduce the numbers of animals needed in life sciences. Thank you for the continued support!
In order to disseminate double resin casting microCT (DUCT) (Hankeova & Salplachta et al eLife 2021), we are happy to present the full protocol in the Journal of Visualized Experiments (JoVE): Hankeova & Salplachta et al JoVE 2021.
On the cover: Our latest paper is out in Cell Reports Methods. We developed a technique to efficiently manipulate gene expression in the embryonic mouse nervous system. We called the technique NETPUNE for neural plate targeting by in utero nanoinjection. Major congratulations to first author Katrin Mangold, PhD student in the lab. The developing mouse embryo is poorly amenable to genetic manipulations that are typically needed for functional gene analysis studies. In this issue, Mangold et al. develop NEPTUNE, a method to rapidly and flexibly transduce the neural plate with gene-expression-modifying viruses prior to neurulation to target the future adult nervous system. The cover image draws parallels between Neptune “the blue planet” in our solar system, Neptune the Roman god of the sea and freshwater, and NEPTUNE the method in which amniotic fluid is injected to control cell-type-specific expression and functional outcomes in brain and spinal cord. Artwork by illustrator Mattias Karlen, cover concept by E.R. Andersson.
We welcome our new postdoc Elisabeth Verboven, who performed her PhD in the Georg Halder Lab at VIB – KU Leuven, Belgium.
We welcome our new postdoc Lenka Belicova, who performed her PhD in the Marino Zerial Lab at the Max Planck Institute of Molecular Cell Biology and Genetics, Germany.
We welcome our new undergraduate student, Anne-Lin de Valk from the University of Applied Sciences in Utrecht, The Netherlands.
ERA Lab contributes to consensus paper on nomenclature for organoids, as HPB Consortium member: Marsee et al, Cell Stem Cell 2021.
Our analysis of gender bias in review processes is out: Andersson et al Front. Res. Metr. Anal. 2021. We find that among the highest-ranked applicants, women with equal quantifiable merits are ranked lower than men with equal merits. Fun anecdote: another high-impact journal rejected this paper, after revisions, because reviewer X “didn’t believe gender bias exists”.
Our latest pre-print is up on bioRxiv! Simona Hankeova and colleagues investigated whether bleeding in Alagille syndrome might be explained by vascular defects pre-disposing to ruptured blood vessels. Using the mouse model we developed, a systematic review of the literature, and retinal fundus photography from patients, we identified multiple previously unrecognized vascular phenotypes and show that vascular frailty could be linked to sparse vascular smooth muscle cells that are highly sensitive to high blood pressure.
Our collaborative paper with Igor Adameyko lab is out in Nature Genetics! Polina Kameneva, Artem V. Artemov and colleagues used multiple approaches to lineage trace neural crest cells and identify sympathoblast lineages of relevance to neuroblastoma. We contributed with in utero transduction and lentiviral barcode lineage tracing of neural crest (Fig 4, see also NEPTUNE technique described in Mangold et al Cell Reports Methods 2021 above). This project was supported by, and is part of, our Knut and Alice Wallenberg project, a collaboration with Igor Adameyko, Jonas Frisén, Niklas Björkström and Ulrika Marklund groups.
Two new arrivals!
We welcome our new postdoc Paulina Zydowicz-Machtel, who performed her PhD in the Jerzy Ciesiołka lab at the Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poland.
We also welcome our new undergraduate student, Luisa Dehandschutter Gortari, from KU Leuven, Belgium.
Two publications from our lab, out on the same day!
- Emma Andersson’s commentary in Science is out (full text link available under The Lab > Publications). In this commentary, Emma discusses two exciting new papers in Science investigating hepatocyte homeostasis and regeneration after injury.
- Our DUCT paper is out in eLife! Simona Hankeova, Jakub Salplachta and colleagues developed a technique to quantify the 3D architecture of the biliary and vascular trees in wild type and Alagille syndrome mice. We show that recovery occurs with distinct architectural mechanisms in hilar and peripheral liver, with resultant architecture that could be severely misinterpreted in 2D sections.
Our latest collaboration is up on bioRxiv! Franziska Hildebrandt in Johan Ankarklev’s lab has led a major effort to apply spatial transcriptomics to large liver lobes. Confirmed zonation of liver functions and previously unreported exciting transcriptionally distinct structures of interest. Noemi Van Hul and Jan Masek from Andersson lab contributing liver and transcriptomic expertise.
We bid farewell and good luck to our postdoc Jan Masek, who is embarking on a new adventure and setting up his own lab at Charles University in Prague!
Sept 30, 2020
Andersson lab is awarded a Knut and Alice Wallenberg project grant to study lineages and fate choices accross organ systems during development. This major project grant is a collaboration with Igor Adameyko, Niklas Björkström, Jonas Frisén and Ulrika Marklund. We are humbled and grateful to the KAW Foundation and the reviewers – and so excited to do this project!
One publication out, and one deposited in bioRxiv!
- Our collaborative project with Jozef Vecera and Jiri Pachernik is out in Stem Cell Research.
- PhD student Katrin Mangold has developed a technique to study gene function in embryonic development more rapidly than is possible with conditional knockouts. NEPTUNE: neural plate targeting by in utero nanoinjection. Here it is, on bioRxiv. 95% of cells in brain are transduced, conditional expression can be acheived with mini-Promotors, and we obtained a really interesting phenotype knocking down Sptbn2… Importantly, this can also reduce the numbers of mice used in science dramatically.
Andersson Lab is awarded the Swedish Foundation’s Starting Grant – an equivalent to the European Research Council Starting Grant. This collaboration between the Erling-Persson Family Foundation, the Kempe Foundations, the Foundation Olle Engkvist Byggmästare, Ragnar Söderberg Foundation and Riksbankens Jubileumsfond welcomes applications from researchers ranked A in the ERC Starting Grant, and recommended for funding, but for whom there was not sufficient funds in the ERC budget.
Read the press release here, and an interview here. We are deeply grateful to all the participating foundations for their initiative, in particular the Ragnar Söderberg Foundation that funded our Swedish Foundation Starting Grant in this round.
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